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Extracted ion chromatograms (EICs) of paraben diagnostic fragment ions in (a–g) DDA and (h–n) DIA data of a pooled <t>follicular</t> fluid sample with a spiking concentration of 25 ng/mL. The diagnostic ion at m / z 123.0082 is not displayed due to its relatively weak peak intensity. The chromatograms shown in DDA correspond to a combination of spectra collected at 10, 20, and 40 eV CEs, while for DIA, a preferred CE (20 or 40 eV) was chosen to demonstrate the best data quality. B1–B6 correspond to the spiked benzophenone standards: B1 – benzophenone-2; B2 – 2,4,4 trihydroxybenzophenone; B3 – 4-hydroxybenzophenone; B4 – benzophenone-1; B5 – dioxybenzone; and B6 – benzophenone-6.
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Extracted ion chromatograms (EICs) of paraben diagnostic fragment ions in (a–g) DDA and (h–n) DIA data of a pooled <t>follicular</t> fluid sample with a spiking concentration of 25 ng/mL. The diagnostic ion at m / z 123.0082 is not displayed due to its relatively weak peak intensity. The chromatograms shown in DDA correspond to a combination of spectra collected at 10, 20, and 40 eV CEs, while for DIA, a preferred CE (20 or 40 eV) was chosen to demonstrate the best data quality. B1–B6 correspond to the spiked benzophenone standards: B1 – benzophenone-2; B2 – 2,4,4 trihydroxybenzophenone; B3 – 4-hydroxybenzophenone; B4 – benzophenone-1; B5 – dioxybenzone; and B6 – benzophenone-6.
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Extracted ion chromatograms (EICs) of paraben diagnostic fragment ions in (a–g) DDA and (h–n) DIA data of a pooled <t>follicular</t> fluid sample with a spiking concentration of 25 ng/mL. The diagnostic ion at m / z 123.0082 is not displayed due to its relatively weak peak intensity. The chromatograms shown in DDA correspond to a combination of spectra collected at 10, 20, and 40 eV CEs, while for DIA, a preferred CE (20 or 40 eV) was chosen to demonstrate the best data quality. B1–B6 correspond to the spiked benzophenone standards: B1 – benzophenone-2; B2 – 2,4,4 trihydroxybenzophenone; B3 – 4-hydroxybenzophenone; B4 – benzophenone-1; B5 – dioxybenzone; and B6 – benzophenone-6.
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Extracted ion chromatograms (EICs) of paraben diagnostic fragment ions in (a–g) DDA and (h–n) DIA data of a pooled <t>follicular</t> fluid sample with a spiking concentration of 25 ng/mL. The diagnostic ion at m / z 123.0082 is not displayed due to its relatively weak peak intensity. The chromatograms shown in DDA correspond to a combination of spectra collected at 10, 20, and 40 eV CEs, while for DIA, a preferred CE (20 or 40 eV) was chosen to demonstrate the best data quality. B1–B6 correspond to the spiked benzophenone standards: B1 – benzophenone-2; B2 – 2,4,4 trihydroxybenzophenone; B3 – 4-hydroxybenzophenone; B4 – benzophenone-1; B5 – dioxybenzone; and B6 – benzophenone-6.
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Fig. 2 Cytokines in the pre-ovulatory microenvironment and serum (A) The expression of IL4, IL5, IL13, IL17, IL6, IL10, TNFA and IFNG mRNA isolated from lymphocytes collected from <t>follicular</t> aspirates within the preovulatory microenvironment was assessed using RT-qPCR. Each data point represents an individual subject, data are expressed as mean ± SEM. * p < 0.05, ns, not significant. mRNA level from OW group compared to NW group (Mann-Whitney U Test). (B) The heatmap of cytokines detection in follicular fluids and serum. The levels of the cytokines were measured using Luminex 200 platform
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Image Search Results


Extracted ion chromatograms (EICs) of paraben diagnostic fragment ions in (a–g) DDA and (h–n) DIA data of a pooled follicular fluid sample with a spiking concentration of 25 ng/mL. The diagnostic ion at m / z 123.0082 is not displayed due to its relatively weak peak intensity. The chromatograms shown in DDA correspond to a combination of spectra collected at 10, 20, and 40 eV CEs, while for DIA, a preferred CE (20 or 40 eV) was chosen to demonstrate the best data quality. B1–B6 correspond to the spiked benzophenone standards: B1 – benzophenone-2; B2 – 2,4,4 trihydroxybenzophenone; B3 – 4-hydroxybenzophenone; B4 – benzophenone-1; B5 – dioxybenzone; and B6 – benzophenone-6.

Journal: ACS Measurement Science Au

Article Title: Non-Targeted Analysis Workflow of Endocrine-Disrupting Chemicals in Ovarian Follicular Fluid: Identification of Parabens by Diagnostic Fragmentation Evidence and Additional Contaminants via Mass Spectral Library Matching

doi: 10.1021/acsmeasuresciau.5c00082

Figure Lengend Snippet: Extracted ion chromatograms (EICs) of paraben diagnostic fragment ions in (a–g) DDA and (h–n) DIA data of a pooled follicular fluid sample with a spiking concentration of 25 ng/mL. The diagnostic ion at m / z 123.0082 is not displayed due to its relatively weak peak intensity. The chromatograms shown in DDA correspond to a combination of spectra collected at 10, 20, and 40 eV CEs, while for DIA, a preferred CE (20 or 40 eV) was chosen to demonstrate the best data quality. B1–B6 correspond to the spiked benzophenone standards: B1 – benzophenone-2; B2 – 2,4,4 trihydroxybenzophenone; B3 – 4-hydroxybenzophenone; B4 – benzophenone-1; B5 – dioxybenzone; and B6 – benzophenone-6.

Article Snippet: The follicular fluid samples were collected from 211 female patients receiving reproductive treatment between 2016 and 2017 by ONE Fertility and McMaster University in Burlington, ON, Canada, for the study of the molecular physiology of human granulosa cells (HiREB 11–252-T).

Techniques: Diagnostic Assay, Concentration Assay

(Left side) MS2 profiles of proposed phase II paraben metabolites (sulfates) identified in unhydrolyzed pooled follicular fluid samples: (a) methyl paraben sulfate, (b) ethyl paraben sulfate, (c) propyl paraben sulfate, (d) 4-hydroxybenzoic acid sulfate, (e) 3,4-dihydroxybenzoic acid sulfate, and (f) methyl 3,4-dihydroxybenzoate sulfate. (Right side) Extracted ion chromatograms (EICs) of corresponding free-from chemicals (precursor ions with mass error ± 5 ppm) in hydrolyzed and unhydrolyzed follicular fluid samples: (g) methyl paraben, (h) ethyl paraben, (i) propyl paraben, (j) 4-hydroxybenzoic acid, (k) 3,4-dihydroxybenzoic acid, and (l) methyl 3,4-dihydroxybenzoate.

Journal: ACS Measurement Science Au

Article Title: Non-Targeted Analysis Workflow of Endocrine-Disrupting Chemicals in Ovarian Follicular Fluid: Identification of Parabens by Diagnostic Fragmentation Evidence and Additional Contaminants via Mass Spectral Library Matching

doi: 10.1021/acsmeasuresciau.5c00082

Figure Lengend Snippet: (Left side) MS2 profiles of proposed phase II paraben metabolites (sulfates) identified in unhydrolyzed pooled follicular fluid samples: (a) methyl paraben sulfate, (b) ethyl paraben sulfate, (c) propyl paraben sulfate, (d) 4-hydroxybenzoic acid sulfate, (e) 3,4-dihydroxybenzoic acid sulfate, and (f) methyl 3,4-dihydroxybenzoate sulfate. (Right side) Extracted ion chromatograms (EICs) of corresponding free-from chemicals (precursor ions with mass error ± 5 ppm) in hydrolyzed and unhydrolyzed follicular fluid samples: (g) methyl paraben, (h) ethyl paraben, (i) propyl paraben, (j) 4-hydroxybenzoic acid, (k) 3,4-dihydroxybenzoic acid, and (l) methyl 3,4-dihydroxybenzoate.

Article Snippet: The follicular fluid samples were collected from 211 female patients receiving reproductive treatment between 2016 and 2017 by ONE Fertility and McMaster University in Burlington, ON, Canada, for the study of the molecular physiology of human granulosa cells (HiREB 11–252-T).

Techniques:

Fig. 2 Cytokines in the pre-ovulatory microenvironment and serum (A) The expression of IL4, IL5, IL13, IL17, IL6, IL10, TNFA and IFNG mRNA isolated from lymphocytes collected from follicular aspirates within the preovulatory microenvironment was assessed using RT-qPCR. Each data point represents an individual subject, data are expressed as mean ± SEM. * p < 0.05, ns, not significant. mRNA level from OW group compared to NW group (Mann-Whitney U Test). (B) The heatmap of cytokines detection in follicular fluids and serum. The levels of the cytokines were measured using Luminex 200 platform

Journal: Journal of ovarian research

Article Title: Immune imbalance in the pre-ovulatory follicular microenvironment of overweight and obese women during IVF.

doi: 10.1186/s13048-025-01606-5

Figure Lengend Snippet: Fig. 2 Cytokines in the pre-ovulatory microenvironment and serum (A) The expression of IL4, IL5, IL13, IL17, IL6, IL10, TNFA and IFNG mRNA isolated from lymphocytes collected from follicular aspirates within the preovulatory microenvironment was assessed using RT-qPCR. Each data point represents an individual subject, data are expressed as mean ± SEM. * p < 0.05, ns, not significant. mRNA level from OW group compared to NW group (Mann-Whitney U Test). (B) The heatmap of cytokines detection in follicular fluids and serum. The levels of the cytokines were measured using Luminex 200 platform

Article Snippet: Total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and free fatty acid (FFA) levels in follicular fluid samples and serum were quantified utilizing an enzyme-linked immunosorbent assay (ELISA) reader (Tecan Sunrise; Männedorf, Switzerland) and commercially available kits (Nanjing Jiancheng Bioengineering Institute Co., Ltd., Jiangsu, China).

Techniques: Expressing, Isolation, Quantitative RT-PCR, MANN-WHITNEY, Luminex

Fig. 3 Comparison of concentrations of lipid profiles between follicular fluids (FF) and serum within the same IVF cycle and individual patients. Data points of total cholesterol (TC), triglycerides (TG), low density lipoproteins cholesterol (LDL-C), high density lipoproteins cholesterol (HDL-C) and free fatty acids (FFA) in FF ( ) and serum ( ) obtained from the full cohort are shown for individual patients. The levels of the lipid profiles were measured using ELISA. ** p < 0.01, *** p < 0.001, lipid profile levels from serum compared to follicular fluids (Wilcoxon matched-pairs signed rank test)

Journal: Journal of ovarian research

Article Title: Immune imbalance in the pre-ovulatory follicular microenvironment of overweight and obese women during IVF.

doi: 10.1186/s13048-025-01606-5

Figure Lengend Snippet: Fig. 3 Comparison of concentrations of lipid profiles between follicular fluids (FF) and serum within the same IVF cycle and individual patients. Data points of total cholesterol (TC), triglycerides (TG), low density lipoproteins cholesterol (LDL-C), high density lipoproteins cholesterol (HDL-C) and free fatty acids (FFA) in FF ( ) and serum ( ) obtained from the full cohort are shown for individual patients. The levels of the lipid profiles were measured using ELISA. ** p < 0.01, *** p < 0.001, lipid profile levels from serum compared to follicular fluids (Wilcoxon matched-pairs signed rank test)

Article Snippet: Total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and free fatty acid (FFA) levels in follicular fluid samples and serum were quantified utilizing an enzyme-linked immunosorbent assay (ELISA) reader (Tecan Sunrise; Männedorf, Switzerland) and commercially available kits (Nanjing Jiancheng Bioengineering Institute Co., Ltd., Jiangsu, China).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay